Research Assistant (Gynecologic Cancer Center of Excellence)
Join the HJF Team! HJF is seeking a Research Assistant in the Gynecologic Cancer Translational Research Center of Excellence (GYN-COE) Program located at the Women s Health Integrated Research Center in Annandale, Virginia to support gynecologic cancer biomarker research using advanced techniques in next-generation genomic sequencing (NGS) as well as mass spectrometry-based proteomics is currently available. HJF provides scientific, technical and programmatic support services to CYN-COE. Projects include development and application of high resolution mass spectrometric techniques for differential identification of gynecologic cancer biomarkers from tissue biopsies, proximal fluids and serum collected from cancers of the uterus, ovary and fallopian tube using stable isotope labeled and label-free quantitative proteomics, including the use of data-dependent and data-independent acquisition techniques. This position will be focused on establishing technical developments in mass spectrometry toward improved analytical precision and throughput for assay of clinical samples using mass spectrometry. Responsibilities: Sample preparation for genomics and proteomics including laser capture microdissection microscopy as well as extraction and quantitation of nucleic acids and proteins from tissue and cell line specimens using standardized techniques. Development and standardization of high throughput proteomic sample processing workflows to include isobaric tag and stable isotope labeling techniques, affinity chromatography, sample fractionation and digestion of tissue and cell line specimens. Operation of nanoflow high performance liquid chromatographs and high resolution mass spectrometers following standard operating procedures including preparation of mobile phases, integrated electrospray emitter nanoflow columns packed with stationary phase, preparation of standard peptide and complex peptide digests, generation of calibration standards, instrument calibration and maintenance. Proteomic data analysis to include database searching, compilation of searched data and curation of peptide standard libraries from in-house and publicly available sources. Scientific and general laboratory infrastructure support and upkeep to scientific research staff as needed including maintenance of basic research laboratory supplies and reagent stocks, cleaning and maintenance of laboratory instrumentation and glassware as well as shipping and receiving of study materials. Required Knowledge, Skills, and Abilities: A strong record of research accomplishments, the ability to work in a team environment, excellent oral and written communications skills in English, presentations of scientific results at national meetings, and their publication in peer-reviewed journals are required. Required skills include demonstrated experience in conducting nanoscale liquid chromatography-mass spectrometry, laser capture microdissection, proteomic sample preparation and post-acquisition proteomic bioinformatics analysis. Previous work in molecular biology, cell biology, or biochemistry is highly desired. Minimum Education/Training Requirements: BS in biology, chemistry or physics with demonstrated experience in liquid chromatography-mass spectrometry analysis . Masters degree a plus. Minimum Experience: at least 2 years of hands on experience with liquid chromatography-mass spectrometry analysis. Physical Capabilities: Long periods of standing and sitting; intricate work with hands; carrying of light laboratory equipment. Supervisory Responsibilities/Controls: May provide guidance/training to laboratory staff when directed by senior scientists. Work Environment: Laboratory environment; may involve working with biohazardous materials; may require working evenings and weekends.