High Throughput Next Generation Sequencing Scientist - NIH - Bethesda, Maryland
High Throughput Next Generation Sequencing Scientist - NIH - Bethesda, MarylandKelly Government Solutions is a strategic supplier and business partner to the federal government and its key suppliers. Through our partnership with the National Institutes of Health, Kelly Government Solutions offers administrative, IT, engineering, scientific, and healthcare professionals the opportunity to work with one of the most prestigious research organizations in the world. We are currently seeking a SCIENTIST experienced in HTS and NGS to work with the National Institutes of Health in Bethesda, MD. Bethesda is 8 miles from Washington, DC.This is a long-term contract position which offers:* Competitive salary with comprehensive benefit package* Opportunity to work at NIH, world's foremost medical research center* Learn more about what Kelly can do for you at www.kellygovernmentsolutions.comTASKS/SERVICES. The contractor shall: 1. Serve as team lead to identify, plan and develop/modify the most applicable protocols and experimental methods based on work experience, scientific knowledge, and review of the literature. 2. Independently perform microarray experiments including preparation of RNA and quality control (QC) RNA from tissues or cells, synthesis of fluorescently labeled target cRNA or cDNA, array hybridization and optical scanning of arrays.3. Independently perform clinical sample collection, processing and cryopreservation, sample monitoring, electronic inventory/tracking, and sample distribution. 4. Independently generate libraries for High-Throughput Next Generation Sequencing such as RNA-Seq, immunoSeq for TCR-b. 5. Independently generate all production DNA sequencing templates; prepare growth boxes with media, operate the plasmid DNA purification robot and alternate manual protocol, and perform agarose gel analysis QC of template DNA. 6. Operate and maintain High-Throughput liquid transfer robotics and DNA sequencing instruments. 7. Generate high-quality sequencing libraries, including isolation of highly purified DNA, kinetic shearing, end-pair, adaptor ligation, size-selection of DNA, and sequencing on NGS sequencer. 8. Maintain accurate and detailed records of all laboratory experiments and QC procedures. 9. Skillful in the collection, arrangement, and organization of data to prepare tables, charts, graphs, summaries, and other materials for effective presentation of experimental results and for publications. 10. Calibrate and operate a variety of laboratory instruments used to perform tests, analyses and other laboratory procedures. 11. Play a leadership role in the organization and maintenance of laboratory; check current inventory of supplies in the laboratory and select, order appropriate reagents, buffers, specimens, and other required materials. 12. Coordinate with staff to analyze and interpret data and prepare summaries of laboratory findings to be used in reports and papers to be presented at scientific meetings and publication in scientific journals. 13. Train and teach other laboratory staff a range of laboratory procedures and the use of various types of laboratory equipment. REQUIREMENTS. The contractor must have: 1. Master's degree in Biology or a related discipline.2. Minimum of eight (8) years of experience in a laboratory environment.3. Experience performing High-Throughput Sequencing Next Generation Sequencing and Library Construction.4. Professional knowledge acquired through formal education, training and experiences of the theories and principles of biology.5. Ability to calibrate and operate laboratory instruments sufficient to independently perform recurring tests, analyses and other laboratory procedures.6. Ability to provide summaries, graphs, and other material for use by self/staff for inclusion in reports, scientific presentations and scientific publications.7. Knowledge of guidelines and regulations regarding biohazard constraints and handling of biological material, infectious agents, chemicals and other similar materials.8. Strong verbal and written communications skills.9. Excellent analytical, organizational and time management skills.PLEASE APPLY ONLINENote that the phone number for our NIH branch is not listed. Due to the high volume of inquiries, we regret that we cannot accept phone calls. All qualified candidates will be contacted. About Kelly Services(R) As a workforce advocate for over 70 years, we are proud to have a role in managing employment opportunities for more than one million workers around the globe. We employ 550,000 of these individuals directly with the remaining workers engaged through our talent supply chain network of supplier partners. Revenue in 2015 was $5.5 billion. Visit kellyservices.com and connect with us on Facebook, LinkedIn and Twitter. Kelly Services is an equal opportunity employer including, but not limited to, Minorities, Females, Individuals with Disabilities, Protected Veterans, Sexual Orientation, Gender Identity and is committed to employing a diverse workforce. Equal Employment Opportunity is The Law.