Research Associate, Biochemistry and Molecular and Cellular Biology - Georgetown University Medical
Research Associate, Biochemistry and Molecular and Cellular Biology - Georgetown University Medical Center
Georgetown University Medical Center (GUMC) is an internationally recognized academic medical center whose missions of research, teaching and patient care are carried out with a strong emphasis on community outreach and the Jesuit principle of cura personalis — care of the whole person.
The Research Associate understands the ongoing crosstalk between the Esherichia coli chromosomal initiator protein, DnaA, and acidic phospholipids present within inner membranes. S/he performs advance level research, using various techniques in microbiology, molecular biology, protein purification and biochemistry, in a study that includes allelic replacement or modification of chromosomal genes involved in maintenance of DNA and membrane biosynthesis, and using transduction and CRISPER methods. Reporting to the Lab Manager/Principal Investigator, the Research Associate has duties that include but are not limited to:
- Studies growth profiles of bacterial cells in both rich and minimal media to elucidate the influence of altered membrane compositions on DnaA-mediated DNA replication.
- Assists in the execution of research experiments, developing laboratory skills, and preparation of experiments for lab use.
- Performs methods in biochemistry, molecular biology, and genetics - such as genetic cloning, gene expression, PCR mutagenesis, protein purifications, bacterial strain constructions, and quantitative biochemical assays.
- Provides oversight for the lab, orders supplies, verifies radiation safety requirements and chemical inventory.
- Maintains laboratory glassware and plastic ware and prepares laboratory reagents and media.
- Takes the lead in the absence of the Pl
- Master's degree in biochemistry, biotechnology, and/or molecular biology
- 5 years of experience in the field
- Ability to take action with minimal supervision, work effectively with peers, mentor junior members of the lab, prepare experiments, and provide guidance in support of the research
- Ability to use sound judgement, verifying unusual situations with the PI
- Advanced proficiency in MS Office applications (Word, Excel, PowerPoint), as well as email and web-based applications
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