Molecular Research Technician I

Employer
Clinical Research Management
Location
Silver Spring, MD
Posted
Jun 13, 2017
Closes
Jun 21, 2017
Function
Analyst, Research
Hours
Full Time
Summary The Applicant shall, with broad guidance from the TPOC, either work alone or with a multidisciplinary team of military, civilian and/contractor research scientists and technicians to plan and perform in vitro and in vivo research using molecular biology, biochemistry, structural biology and molecular immunology to generate candidate malaria vaccines/research antigens and investigate immune responses to these antigens in pre-clinical (lab rodents, New Zealand White rabbits, nonhuman primates) and from clinical (human) vaccine trials. RESPONSIBILITIES Antigen discovery/process development/Vaccine Development Perform all steps associated with molecular engineering. Perform all steps associated with protein expression, purification and characterization to include (but not limited to) fermentation, micro fluidization, chromatography, methods for protein quality and quantitation assessment; gels and Western blots, and immunochemistry. Perform all assays associated with immunochemical and immunological characterization of recombinant proteins. Perform specialized humeral assays to include subunit, avidity, and isotyping ELISAs, IFA, Multiplex-based assays. Perform and develop functional assays that determine the biological function of antibodies. Perform research on cellular immune responses using standard techniques such as ELISPOT, Multiplex based assays, Flow-Cytometry and shall perform aseptic tissue culture. Prepare standards and solutions as tasked. Maintain an accurate log of reagents, laboratory supplies and equipment. Perform general laboratory administrative duties. Perform standard animal handling techniques (rodents and nonhuman primates) as required. Prepare oral and poster presentations for presenting at approved conference. May give oral presentations at laboratory meetings. Provide assistance with preparation of progress reports/technical reports and standard operating procedures. Work closely with other Government, Military and Contractor personnel to develop, and gain approval of human and animal use protocols as are required for the performance of this task. Perform laboratory tasks according to established Standard Operating Procedures (SOP's). Establish Standard Operating Procedures (SOP's) for assays. Maintain and update laboratory notebooks and computer electronic files with detailed experimental plans, data and data analysis. Maintain a clean and safe laboratory. It may be necessary for the technician to travel off site to perform experiments. These locations are to be named. Functions may include, but are not limited to assist with immunizations and challenges; discussion of study with off-site scientists; training of off-site investigators on specimen collection and processing, performing challenges; learning techniques at offsite laboratories; and/or retrieval of specimens and documents. Maintain and operate Government owned equipment (ELISA plate washers and readers, BIAcore affinity measurement equipment (such as the Attana), flow cytometers, cell counters, cell sorters, array reader, micro fluidizer, fermenter, centrifuges, imaging systems, microscopes) Maintain a safe workplace ensuring that he/she is aware of and observes appropriate safety and occupational health rules and regulations. Required to attend institutional safety training and report any infractions of safety procedures to the facility Safety Officer. The Applicant shall maintain an appropriate, professional demeanor at all times and be able to work as a team member. MINIMUM QUALIFICATIONS BS Degree in Biological Sciences Must have animal experience in a lab setting Basic Familiarity with technical lab tasks Technical skills and knowledge of molecular, biochemical, malaria parasitology or immunological assays. #CB Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities The contractor will not discharge or in any other manner discriminate against employees or applicants because they have inquired about, discussed, or disclosed their own pay or the pay of another employee or applicant. However, employees who have access to the compensation information of other employees or applicants as a part of their essential job functions cannot disclose the pay of other employees or applicants to individuals who do not otherwise have access to compensation information, unless the disclosure is (a) in response to a formal complaint or charge, (b) in furtherance of an investigation, proceeding, hearing, or action, including an investigation conducted by the employer, or (c) consistent with the contractor's legal duty to furnish information. Qualifications Skills Behaviors Motivations Education Experience Licenses & Certifications