Molecular Antiparasitic Technician (WRAIR-In Vitro Core)

Silver Spring, MD
May 02, 2017
Aug 17, 2017
Specialty Trades
Full Time
GDIT is seeking a Molecular Antiparasitic Technician (In Vitro Core) to support the mission of the Experimental Therapeutics (ET) Branch of the Walter Reed Army Institute of Research (WRAIR). The mission of the WRAIR Branch of Experimental Therapeutics is to discover and develop new pharmaceutical agents to protect military members from death, disease, and injury. The ET Branch is organized into three distinct departments which contribute to the different phases leading to FDA drug approval. The three comprising departments are the Departments of Drug Discovery, Drug Development, and Clinical Pharmacology.

This position is in direct support of the Department of Drug Discovery. Specific tasks include:

    Provide in vitro and in vivo screening support for antiparasitic drug discovery.Transfect multiple strains of Leishmania promastigotes with a luciferase expressing vector.Establish individual clones of transfected parasites with the characteristic that only promastigotes stably expressing luciferase activity (without any antibiotic selection pressure) are maintained.Establish a high throughput in vitro screen to be used screening compounds for efficacy against Leishmania species.Execute in vitro screen using multiple strains of Leishmania and multiple stages.Assay individual clones to determine any potential differences in luciferase expression and growth rates.Infect macrophages with the labeled promastigotes to generate amastigotes.Perform drug screen assays to quantify current and screen new drugs with antileishmanial activity.Provide the Leishmania cultures required to infect animal models in order to study the parasite progression through the animal.Attend and present at relevant national scientific conferences, if required by the Department of Discovery.Analyze and summarize experimental data for reports and presentations and publications.Maintain a safe working environment and adhere to applicable safety regulations.Screen compounds against Plasmodium falciparum blood stage.Maintain an inhibitor database to record all results from blood stage assay.Inventory parasite cryobanks and maintain records for future reference.

Physical Capabilities: Physical Capabilities: Work may involve long periods of standing and handling of BSL2 level infectious agents, toxic chemicals, and blood-borne pathogens. Work will require the ability to operate research equipment requiring manual dexterity.

Work environment: Biosafety Level (BSL) 2 laboratory and animal BSL 2 laboratory. Potential exposure to infectious agents, toxic chemicals, and blood-borne pathogens. Must be able to wear appropriate personal protective equipment. Will also work in an office setting.

Education MS in Microbiology, biomedical field or related field from an accredited university.


Qualifications 2-5 years of related experience in scientific research, analysis and/or engineering.

  As a trusted systems integrator for more than 50 years, General Dynamics Information Technology provides information technology (IT), systems engineering, professional services and simulation and training to customers in the defense, federal civilian government, health, homeland security, intelligence, state and local government and commercial sectors.With approximately 32,000 professionals worldwide, the company delivers IT enterprise solutions, manages large-scale, mission-critical IT programs and provides mission support services.GDIT is an Equal Opportunity/Affirmative Action Employer - Minorities/Females/Protected Veterans/Individuals with Disabilities.