Molecular Antiparasitic Technician (WRAIR-In Vitro Core)
GDIT is seeking a Molecular Antiparasitic Technician (In Vitro Core) to support the mission of the Experimental Therapeutics (ET) Branch of the Walter Reed Army Institute of Research (WRAIR). The mission of the WRAIR Branch of Experimental Therapeutics is to discover and develop new pharmaceutical agents to protect military members from death, disease, and injury. The ET Branch is organized into three distinct departments which contribute to the different phases leading to FDA drug approval. The three comprising departments are the Departments of Drug Discovery, Drug Development, and Clinical Pharmacology.This position is in direct support of the Department of Drug Discovery. Specific tasks include:
- Provide in vitro and in vivo screening support for antiparasitic drug discovery.Transfect multiple strains of Leishmania promastigotes with a luciferase expressing vector.Establish individual clones of transfected parasites with the characteristic that only promastigotes stably expressing luciferase activity (without any antibiotic selection pressure) are maintained.Establish a high throughput in vitro screen to be used screening compounds for efficacy against Leishmania species.Execute in vitro screen using multiple strains of Leishmania and multiple stages.Assay individual clones to determine any potential differences in luciferase expression and growth rates.Infect macrophages with the labeled promastigotes to generate amastigotes.Perform drug screen assays to quantify current and screen new drugs with antileishmanial activity.Provide the Leishmania cultures required to infect animal models in order to study the parasite progression through the animal.Attend and present at relevant national scientific conferences, if required by the Department of Discovery.Analyze and summarize experimental data for reports and presentations and publications.Maintain a safe working environment and adhere to applicable safety regulations.Screen compounds against Plasmodium falciparum blood stage.Maintain an inhibitor database to record all results from blood stage assay.Inventory parasite cryobanks and maintain records for future reference.