Research Specialist or Postdoctoral Associate-Imaging, MultiFISH Project
About HHMIHoward Hughes Medical Institute (HHMI) is a science philanthropy whose mission is to advance biomedical research and science education for the benefit of humanity. We empower exceptional scientists and students to pursue fundamental questions about living systems, and work to share scientific discoveries with researchers, students, and science-curious individuals around the world. Founded in 1953 by aviator and industrialist Howard R. Hughes, HHMI is headquartered in Chevy Chase, Maryland, and employs more than 2,500 people across the US Visit hhmi.org/careers to learn more about working at HHMI. About JaneliaJanelia Research Campus is a pioneering research center in Ashburn, Virginia, where scientists pursue fundamental questions in neuroscience and imaging. The Howard Hughes Medical Institute (HHMI) launched Janelia in 2006, establishing an intellectually distinctive environment for scientists to do creative, collaborative, hands-on work. The Janelia Research Campus is a unique, world-class research institute with a major focus on the development of cutting-edge imaging and analysis tools. We prize collaboration and in addition to supporting independent laboratories, we support a unique ecosystem of Project Teams to tackle experimentally challenging problems at scale. To learn more about working at Janelia, visit janelia.org/careers. SummaryThe Howard Hughes Medical Institute's Janelia Research Campus is looking for an outstanding Research Specialist or Postdoctoral Associate interested in developing new methods for highly multiplexed imaging of RNA in neural tissue, using the Expansion Microscopy technique for physically expanding tissue by embedding in a swellable gel. The MultiFISH project team is a new effort to combine new molecular and imaging methods in order to develop a robust protocol for multiplexed RNA Fluorescence In Situ Hybridization (FISH). This protocol will be made readily available to research groups at Janelia and in the broader neuroscience community. The broader goal is to enable studies that combine in vivo experiments with post-fixation molecular characterization of the same tissue. We seek a candidate interested in developing new methods and helping to develop a robust protocol including tissue handling, probing, and imaging. This is a highly collaborative position with opportunities to interact with the Tillberg, Sternston, Svoboda and Lavis Labs. Individuals interested in pursuing an academic research career or a specialist with experience in histology and/or optical imaging are encouraged to apply. Principal Responsibilities:Develop cutting edge hydrogel tissue embedding methods.Carry out RNA Fluorescence In Situ Hybridization (FISH), high content imaging, and spectral unmixing in thick brain tissue slices.Work collaboratively with a team of scientists to develop a protocol for multiplexed RNA FISH in thick tissue slices suitable for routine use in neuroscience experiments. Preferred Qualifications:PhD in Engineering, Bioengineering, Biology, Neuroscience or a related disciplineStrong background in optical imaging and image processing using large imaging datasetsExperience with RNA Fluorescence In Situ Hybridization (FISH) useful but not necessaryAbility to work as part of a teamAbility to develop novel approaches HHMI is an Equal Opportunity Employer.