Process Lead - FlyLight Team Project
About HHMI:Howard Hughes Medical Institute (HHMI) is a science philanthropy whose mission is to advance biomedical research and science education for the benefit of humanity. We empower exceptional scientists and students to pursue fundamental questions about living systems, and work to share scientific discoveries with researchers, students, and science-curious individuals around the world.Founded in 1953 by aviator and industrialist Howard R. Hughes, HHMI is headquartered in Chevy Chase, Maryland, and employs more than 2,500 people across the US Visit hhmi.org/careers to learn more about working at HHMI. About Janelia:Janelia Research Campus is a pioneering research center in Ashburn, Virginia, where scientists pursue fundamental questions in neuroscience and imaging. The Howard Hughes Medical Institute (HHMI) launched Janelia in 2006, establishing an intellectually distinctive environment for scientists to do creative, collaborative, hands-on work. To learn more about working at Janelia, visit janelia.org/careers. Summary: FlyLight team project is a large-scale interdisciplinary research project aimed at mapping the Drosophila brain using a high throughput imaging approach on lines genetically engineered to express fluorescent proteins in only a subset of neurons. The brains and central nerve cords of these flies are dissected, processed through immunohistochemistry, and then imaged with confocal microscopy. Ultimately, these images are used to build up models of the fly central nervous system that describe cell morphology, connectivity and lineage in the adult and developing fly. These tools and models will be shared with the scientific community worldwide to advance the understanding of the structure, development and function of the fly nervous system.The process lead performs professional work in support of scientific research, including: Drosophila brain and CNS dissections and mounting, immunohistochemistry and confocal microscopy imaging. In addition, the process lead combines technical excellence with high-throughput project planning, leadership and guidance of laboratory technicians, and interaction and collaboration with multiple research and support teams.Essential Duties and Responsibilities:Dissect, stain and mount Drosophila brains and ventral nerve cords (adult and larval). All steps must be performed at consistently high quality according to established protocols.Image Drosophila brain and ventral nerve cords using Zeiss LSM700; LSM710 and LSM780 confocal microscopes.Train and guide research technicians to perform multiple tasks while planning and coordinating the team's day-to-day schedule.Quality control of confocal images and any other aspect of the workflow.Keep meticulous records, document work effort, schedule and estimate time for upcoming projects, and prepare reports.Communicate effectively with the manager, team members, researchers, and other support groups at Janelia.Demonstrate proactive and flexible problem solving.Report on the production process output and microscope performance on a weekly and monthly basis.Participate in project meetings and planning sessions. Preferred Qualifications:Master's degree, PhD, or equivalent education and experience in the life sciences.Experience in tissue preparation and confocal microscopy (experience with Carl Zeiss confocal microscopes preferred).Experience in Drosophila Biology / Neurobiology is a major plus.Demonstrated ability to work in a team, meeting or exceeding predetermined scientific goals and milestones.Previous experience in project management is a plus. Skills and Abilities:Expert knowledge and hands-on experience in immunohistochemistry and confocal imagingExcellent interpersonal skills to communicate efficiently with colleaguesStrong organizational skills to develop and optimize project schedules and team planningCommitment to the team's goal of providing outstanding technical support for research projects Physical Requirements:Excellent vision (correction with glasses or contacts is fine)Fine motor skills to manipulate tissue samples.Ability to sit for several hours at a microscope (with breaks) Additional Information:May maintain insects, plants, animals and/or bacterial and tissue cultures for experimental purposes. HHMI is an Equal Opportunity Employer.