Research Technologist

Johns Hopkins University
Baltimore, MD
Oct 01, 2022
Oct 10, 2022
Analyst, Research
Full Time
We are looking for an enthusiastic and self-motivated Research Technologist who is highly organized, to join the Goff Laboratory in developing and utilizing cutting-edge, high throughput genetic, genomic and computational technologies for the exploration of neural cell fate decisions during brain and ENS development. The successful candidate will support several current and future projects using high-throughput single cell genomic and transcriptomic assays, as well as spatial transcriptomic assays and will provide support for the Goff laboratory in ongoing research projects and general laboratory functions. After entry-level training under the general supervision of the faculty investigator, the individual will be responsible for performing routine molecular biology assays and contributing to general maintenance of the laboratory, inventory, equipment, and supplies. The candidate will also present summaries and quality assessments of data generated at regular intervals.Specific Duties & Responsibilities:Work on high throughput molecular biology: DNA and RNA purification, library preparation for single cell sequencing and spatial transcriptomics.Develop and apply microfluidics techniques for gradient cell culture systems.Analyze data using a variety of utilities, including Python, R and machine learning algorithmsFollow established procedures or protocols to perform experiments. Work with large datasets will be required, and strong computational skills are desired.Prepare solutions and reagents, order materials and supplies, maintain sterile ready-to-use glassware, keep inventories of lab stocks, and maintain laboratory equipment.Careful record keeping of all experiments performed and assessment of quality of data generated is critical.Collect, record, organize and verify the accuracy of data; perform calculations and prepare charts, graphs and reports. May use software applications to assemble, manipulate and/or format data and test reports.Perform miscellaneous related duties as assigned.Essential Job Functions: Maintenance and operation of basic laboratory equipmentOrder chemicals, reagents, and other laboratory supplies. Manage laboratory inventory and stocksManaging and compliance with laboratory safety standardsPerform single cell isolation and RNA-Seq library capture for laboratory and collaborative projectsInteract and engage with other lab members in a productive and professional mannerManagement of and work with mouse colonies.Participate in laboratory meetings and planning sessions. Read literature applicable to research areas as required. Perform the duties of a Laboratory Assistant as required.Minimum Qualifications (Mandatory):Bachelor's Degree in Biology, Chemistry, or related field.* Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date. Preferred Qualifications:MS in Molecular/Cellular Biology, Developmental Biology, Neuroscience, or Biomedical Engineering A working knowledge of one or more of the above disciplines achieved through 3+ years of direct laboratory experience.Formal coursework or laboratory experience in genetics, molecular biology, and/or neuroscience techniques would be highly desirable.2+ years of independent research experience or experience with NGS library preparation, PCR, RNA techniques.Experience with microfluidics assay design, production, and application.Prior experience with assay development and automation.Basic command-line proficiency (eg, working knowledge of Linux, Python, R) Familiar with scientific software for analyzing and reporting data.Have an eye for experimental details, maintain careful records, work independently, communicate clearly and be eager to learn new techniques.Experience with single cell RNA-Seq, spatial transcriptomics, or microfluidic cell culture systems.Programming experience or experience with linux systems and command-line utilities.Special Knowledge, Skills & Abilities:Basic understanding of molecular biology and genetics.Ability to safely operate scientific laboratory equipment.Must be highly collaborative, team-oriented. Must have excellent communication skills to succeed in working with a diverse and inclusive team and assist post-doctoral fellows and graduate students by performing various investigative procedures and operating a variety of instruments and other scientific equipment.Physical Requirements:Sitting in a normal seated position for extended periods of time. Reaching by extending hand(s) or arm(s) in any direction. Finger dexterity to manipulate objects with fingers rather than with whole hand or arm, for example, using a keyboard. Classified Title: Research TechnologistRole/Level/Range: ACRO40/E/02/CC Starting Salary Range: $15.00 - $20.00/hr (commensurate with experience)Employee group: Full Time Schedule: M-F, 8:30 to 5pm Exempt Status: Non-Exempt Location: School of Medicine Campus Department name: SOM Genetics Institute of Gen Medicine Personnel area: School of MedicineThe successful candidate(s) for this position will be subject to a pre-employment background check.If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the Talent Acquisition Office at For TTY users, call via Maryland Relay or dial 711.Johns Hopkins has mandated COVID-19 and influenza vaccines, as applicable. Exceptions to the COVID and flu vaccine requirements may be provided to individuals for religious beliefs or medical reasons. Requests for an exception must be submitted to the JHU vaccination registry. For additional information, applicants for SOM positions should visit and all other JHU applicants should visit following additional provisions may apply, depending on campus. Your recruiter will advise accordingly.The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.Note: Job Postings are updated daily and remain online until filled.Equal Opportunity EmployerAll qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.EEO is the LawLearn more: of Medicine - East Baltimore Campus

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