Research Associate II-Molecular & Cell Biology

Frederick National Laboratory for Cancer Research
Frederick, MD
Sep 26, 2022
Oct 01, 2022
Analyst, Research
Full Time
Research Associate II-Molecular & Cell Biology Job ID:req2826Employee Type: exempt full-timeDivision:Laboratory Animal Science ProgramFacility:Frederick: Ft DetrickLocation:PO Box B, Frederick, MD 21702 USA The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc. The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases. Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.PROGRAM DESCRIPTION The Laboratory Animal Sciences Program (LASP) provides exceptional quality animal care and technical support services for animal research performed at the National Cancer Institute at the Frederick National Laboratory for Cancer Research. The Center for Advanced Preclinical Research (CAPR) within LASP developspreclinical model systems for evaluation of cancer therapeutics and biomarker discovery, facilitating the translation of research findings to the clinic. Mouse models are developed or retooled for application to studies assessing small molecule drugs, immunotherapy, and other cutting-edge cancer treatments. The successful candidate will be engaged inin vitrobiomarker studies evaluating a variety of experimental anticancer agents in tissues derived from mouse models of cancer, as a member of CAPR's Preclinical Evaluation team. The RAII will perform a key role in laboratory studies supporting translational research, by analyzing the molecular and pathway effects of experimental therapeutics in mouse models for cancer. The RAII will also assist in the collection, preparation, analysis and storage of tumor tissue samples as well as initiate primary tumor cell culture. Duties may include basic cell and molecular laboratory work such as cell line maintenance and animal genotyping, and responsibility for lab maintenance and writing SOPs. He/she will maintain strong working relationships with other team members as well as with CAPR collaborators.KEY ROLES/RESPONSIBILITIESConduct experimental assays on tumor tissues and cellsPerform molecular biology procedures such as nucleic acid extraction and purification, PCR, and Western blotsEstablish primary tumor cell lines from mouse and human tumor tissueMaintain cancer cell lines in culture and perform drug sensitivity assays on cancer cell linesViral infection and transient transfection of cell linesMay implement and troubleshoot new protocolsModify and update protocols as neededExecute experiments independently with guidance from program scientistsMaintain proper quality control checks for experiments, assays, and equipment. General laboratory upkeep.Compile, analyze, and report scientific data to program scientistsKeep accurate and detailed records and documents laboratory operations and data collection.Computer/data management, including maintenance of complex Excel spreadsheets, GraphPad Prism software.Perform assignments independently and following all applicable regulations, policies, and proceduresWork involves the use of potentially hazardous material or may be located in areas where such materials are used.Work may require the use of personal protective equipment and requires adherence to strict safety protocols.Schedule may include occasional weekend and holiday hours.BASIC QUALIFICATIONSTo be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:Possession of a Bachelor's degree from an accredited college/university in a field related to biomedical research or four (4) years equivalent experience in the appropriate field in lieu of degree. Foreign educated candidates who have completed part or all of their education outside of the United States must have their foreign education evaluated by a company-approved accrediting organization to assure that it has met the equivalency of the qualifications of degree work in the United States.In addition to educational requirements, a minimum of five (5) years job-related biomedical experience.Ability to obtain and maintain a security clearance.PREFERRED QUALIFICATIONSCandidates with these desired skills will be given preferential consideration:Expertise inmolecular biology procedures such as nucleic acid extraction and purification, PCR, and Western blots.Experience with primary tissue culture and cell line maintenance.Cancer biology experience preferred but not essential.Excellent organizational skills.Experience with CRISPR methodology a plus but not required.Experience with flow cytometry a plus but not required.JOB HAZARDSThis position is subject to working with or have potential for exposure to animal(s) and/or animalmaterial(s).This position is subject to working with or have potential for exposure to infectious material, requiringmedical clearance and immunizations.This position may require continuous or intermittent exposure to and exertion in environmentalconditions such as working outdoors, temperature extremes, humidity extremes. etc. as part of the job. Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)#readytowork

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